Influx

BD Influx

The BD Influx™ Cell Sorter is a highly modular, stable stream-in air flow cytometry cell sorter equipped with 4 air-cooled lasers. It allows for high-speed multicolour sorting with acquisition rates of up to 200,000 events per second.

The Influx sorter is able to sort into two- and four-way bulk sorting devices for a variety of tube sizes or can sort using the Cell Deposition Unit (CDU) to multi-well plates or microscope slides. To support aseptic sorting, exchangeable fluidics allow the operator to easily replace the sample line or the complete fluidics path, from sheath tank to nozzle tip (extra costs covered by the investigator). The Influx sorter uses SPIGOT software for acquisition.

Configuration of the BRC INFLUX sorter

BRC INFLUX 1 (flow cytometry laboratory operated):

4 laser, 14 parameter instrument (405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red)), allows simultaneous use of all lasers. CDU and Aerosol management system installed. Laser/Detector and filter configuration: 5 Blue/3 Red/2 Violet /4 Yellow-Green).

Laser	DLP Mirror	BP Filter	Intended Dye
488 nm Optional	550 nm	530/40 nm	FITC
	600 nm	580/30 nm	PE
	645 nm	610/20 nm	PE-TexasRed/PI
	740 nm	750 nm	PE-Cy7
		488/10 nm	SSC
	645 nm	692/40 nm	PE-Cy5.5
	527 nm	530/30 nm	EGFP
	527 nm	542/27 nm	YFP
561 nm Optional	700 nm	750 nm	PE-Cy7
	645 nm	670/30 nm	PE-Cy5
	600 nm	610/20 nm	PE-TexasRed
	600 nm	585/29 nm	PE
	610 nm	593/40 nm	DsRed
	610 nm	624/40 nm	mCherry
	685 nm	710/50 nm	Pe-Cy5.5
633 nm	740 nm	750 nm	APC-Cy7
	700 nm	720/40 nm	Alexa700
		670/30 nm	APC
405 nm	480 nm	460/50 nm	PacificBlue
405 nm	480 nm	520/35 nm	AmCyan

Note
Optional means: The core laboratory owns these filters as well, but they are not routinely installed in the cytometer. If a user requires them for an experiment, please inform FCL staff in advance.

Booking

This machine is exclusively operated by flow cytometry laboratory staff.

The instrument and operators are available for sorts Monday - Friday 10am - 5pm.

All sorts are run with sterile 1x PBS as sheath buffer. Users can provide their own sheath buffer if necessary but must inform the low core laboratory staff at least 2 days in advance.

The sorts are scheduled on a first-come, first-served basis. Members of the BRC's Faculty of Translational Medicine will be given priority.

The flow cytometry laboratory staff will assign sorting times and strive to avoid large blocks of unused time to maximise access. Users will be able to view the booking calendar.

To book sorting time on this instrument, please email (BRCFlowCore@gstt.nhs.uk) a completed sort booking form (word) to the flow laboratory team.

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